Table 1 Screening of differentially expressed genes through joint analysis of multiple omics, followed by Rfp and orf224 for yeast experiment verification
Transcription ID | Protein. accession | Protein interaction | log2FC | p-value | Description | |
|---|---|---|---|---|---|---|
orf224 | Rfp | |||||
RNA editing protein | ||||||
 BnaA09g06660D | A0A078FUQ6 | + | – | −0.61706 | 0.00150 | RNA recognition motif (RRM)-containing protein |
 BnaC06g23390D | A0A078FDM2 | – | + | −0.23610 | 0.53657 | RNA polymerase II subunit C-terminal domain phosphatase |
 BnaA01g22260D | A0A078GHA1 | – | – | − 1.22769 | 0.01624 | RNA-binding protein 25 isoforms X1 |
Respiratory electron transport chain protein | ||||||
 BnaA02g35610D | A0A078JFK2 | – | + | −0.79086 | 0.00062 | Electron transfer activity/cupredoxin superfamily protein |
 BnaA05g32320D | A0A078I0S7 | – | + | 0.73948 | 0.16802 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 6 |
 BnaA06g16370D | A0A078F502 | – | – | 0.98176 | −0.00616 | Gamma carbonic anhydrase-like 2 |
 BnaC03g14740D | A0A078I202 | + | – | −1.16106 | 0.00038 | SPFH/Band 7/PHB domain-containing membrane protein |
 BnaC08g43300D | A0A078GBF1 | – | – | −0.87303 | 0.00190 | Encodes UDP-d-apiose synthase |
 BnaC09g47460D | A0A078G848 | – | + | −1.00000 | 0.00098 | NADH dehydrogenase [ubiquinone] flavoprotein 1 |
Anther development protein | ||||||
 BnaA01g13540D | A0A078ICM7 | + | – | −1.88014 | 0.00328 | UDP-glucose 4-epimerase 2 |
 BnaA02g05650D | A0A078F7X9 | – | + | 0.62574 | 0.00412 | Pollen Ole e 1 allergen and extensin family protein |
 BnaA04g21720D | A0A078FVE9 | – | – | − 4.52980 | 0.00001 | Type III polyketide synthase A-like |
 BnaA08g15000D | A0A078GE34 | – | – | −6.67917 | 0.00010 | Tetraketide alpha-pyrone reductase 1-like |
 BnaC07g33620D | A0A078F9K9 | + | – | 0.83443 | 0.06855 | Pollen-specific leucine-rich repeat extension-like protein 1 |
 BnaA04g26600D | A0A078HGU6 | + | – | −1.34373 | 0.00096 | LDL receptor wingless signaling/trafficking chaperone |
Energy transport-related protein | ||||||
 BnaA03g01500D | A0A078FEE1 | – | – | −1.18473 | 0.00094 | Lysosomal beta glucosidase-like |
 BnaA10g11120D | A0A078HLN1 | – | – | −1.42526 | 0.00070 | Calcium-transporting ATPase |
 BnaC01g21970D | A0A078H9K8 | – | – | −0.64160 | 0.04709 | Dihydrolipoyl dehydrogenase |
BnaC06g00540D | A0A078IEJ9 | – | – | 2.38727 | 0.00002 | Probable mitochondrial chaperone BCS1-B |
 BnaCnng66500D | A0A078JT79 | – | – | 0.88088 | 0.00126 | ATP synthase subunit delta, chloroplastic-like |
Tapetum development protein | ||||||
 BnaA01g23670D | A0A078FYQ2 | + | – | −3.60823 | 0.00012 | May play a role in tapetum development. |
 BnaA09g10680D | A0A078HQA1 | + | – | −5.68514 | 0.00001 | 4-Coumarate-CoA ligase-like 1 |
Oxidative phosphorylation protein | ||||||
 BnaA01g17520D | A0A078H3Z4 | – | + | −2.71786 | 0.00052 | Peroxidase |
 BnaC01g19630D | A0A078GUT8 | – | – | 1.71357 | 0.00002 | NADPH-protochlorophyllide oxidoreductase |