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Fig. 2 | BMC Plant Biology

Fig. 2

From: SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress

Fig. 2

Expression patterns of SiFBA5 under cold stress and its subcellular localization. a qRT-PCR analysis of SiFBA5 in S. involucrata. Means ± SD was obtained from three biological replicates. *, P < 0.05; **, P < 0.01. b Subcellular localization of SiFBA5 in tobacco epidermal cells. SiFBA5-GFP and empty control vector (35S::GFP) were transiently expressed in tobacco epidermal cells. Fluorescent images were taken by confocal microscopy after Agrobacterium-mediated infiltration for 48 h. Upper row, the control panel of 35S::GFP, lower row, 35S::SiFBA5-GFP. From left to right: channels of GFP fluorescence, bright field, chloroplast autofluorescence and an overlay of all three. Scale bar = 20 μm. One-way ANOVA was used to compare the statistical difference between measurements (P < 0.05)

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