Table 4 Method validation parameters for the quantification of colchicine

1

Instrument precision [%CV, n=10]

0.44

2

Repeatability [%CV, n=10]

0.58

3

Linearity range (ng/spot; n=12^a

1000–5000

4

Correlation coefficient (r²)

0.975

5

Regression equation (Linear)

Y= 9178+ 4.527*X

6

Calculated SD value (CATS software)

10.27

7

^bLimit of detection (LOD) (ng) [3 x SD/S] (ng/band)

70

8

^bLimit of quantitation (LOQ) (ng) [10 x SD/S] (ng/band)

210

9

R_f and λmax

0.27; 254 nm