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Table 3 Box Behnken Design (BBD) with four response variables: Microwave power (A), Irradiation Time (B), Solvent composition (C) and pH (D)

From: Screening the elite chemotypes of Gloriosa superba L. in India for the production of anticancer colchicine: simultaneous microwave-assisted extraction and HPTLC studies

Run

Response variables

Colchicine content (mg/g) dry biomass

Microwave power (W)

Irradiation Time (min)

Solvent composition (% aqueous ethanol)

pH

Experimental

Predicted

1

300

9

30

4

5.920

5.703

2

450

6

30

4

7.370

7.037

3

450

6

30

4

7.410

6.865

4

450

6

40

6

5.732

6.272

5

600

3

30

4

5.836

5.953

6

450

3

30

2

6.583

6.818

7

300

6

30

2

5.980

5.486

8

600

6

40

4

6.156

6.534

9

450

9

30

6

6.063

5.917

10

600

6

20

4

6.230

6.448

11

450

6

20

2

6.780

6.401

12

300

6

20

4

5.849

5.919

13

450

9

30

2

6.624

6.340

14

300

6

40

4

5.755

6.346

15

600

9

30

4

6.159

5.969

16

600

6

30

2

6.560

6.540

17

600

6

30

6

5.456

5.917

18

300

6

30

6

5.240

5.622

19

450

3

40

4

6.110

5.729

20

450

6

30

4

7.340

6.557

21

300

3

30

4

5.362

5.598

22

450

6

40

2

6.843

6.654

23

450

3

20

4

6.234

5.917

24

450

3

30

6

5.354

5.831

25

450

6

20

6

5.980

6.861

26

450

9

20

4

6.612

6.248

27

450

9

40

4

6.543

6.586