Table 1 Plackett-Burman design and analysis of variance (ANOVA) for experimental results for extraction of colchicine
Std. order | Microwave power (Watt) | Irradiation time (min) | Particle size (mm) | Solvent composition (%aqueous) | Solid: solvent | pH | Extraction steps | Colchicine (mg/g dry wt.) | Predicted |
|---|---|---|---|---|---|---|---|---|---|
1 | 600 | 3 | 1 | 20 | 15 | 2 | 2 | 6.104 | 6.11 |
2 | 600 | 9 | 0.5 | 40 | 15 | 2 | 1 | 4.980 | 5.01 |
3 | 300 | 9 | 1 | 20 | 30 | 2 | 1 | 6.660 | 6.488 |
4 | 600 | 3 | 1 | 40 | 15 | 6 | 1 | 2.824 | 2.88 |
5 | 600 | 9 | 0.5 | 40 | 30 | 2 | 2 | 5.041 | 5.007 |
6 | 600 | 9 | 1 | 20 | 30 | 6 | 1 | 5.304 | 5.476 |
7 | 300 | 9 | 1 | 40 | 15 | 6 | 2 | 2.824 | 2.492 |
8 | 300 | 3 | 1 | 40 | 30 | 2 | 2 | 3.624 | 3.885 |
9 | 300 | 3 | 0.5 | 40 | 30 | 6 | 1 | 1.864 | 1.877 |
10 | 600 | 3 | 0.5 | 20 | 30 | 6 | 2 | 4.344 | 4.102 |
11 | 300 | 9 | 0.5 | 20 | 15 | 6 | 2 | 3.390 | 3.721 |
12 | 300 | 3 | 0.5 | 20 | 15 | 2 | 1 | 5.224 | 5.120 |