Fig. 2

Molecular characterization of eight independent switchgrass lines containing the pShOMT::QsuB construct. a Detection of the QsuB gene by PCR. ‘A4’ is a gDNA sample from wild-type switchgrass and ‘Plasmid’ is the pShOMT::QsuB construct used for plant transformation. b Detection of QsuB transcripts by RT-qPCR. QsuB expression levels relative to that of PvUBQ6 are shown. cDNA obtained from a line containing the pShOMT::GUS construct were used as negative control. Values are means ±SD of two biological replicates (n = 2)