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Table 1 The sequences of the primers used in this work for vector construct, screening the positive transform, and transcript assay

From: Heterologous expression of Arabidopsis thaliana rty gene in strawberry (Fragaria × ananassa Duch.) improves drought tolerance

Gene

Accession

Primer Sequence (5′ → 3′) a

Protein

Ortholog gene in Arabidopsis

Function

Reference (s)

rty

AY050987

F: AAGCTTATGAGC GAAGAACAACCACACGC

R: GAGCTCTTACAT TTCGAGATTATTATCACTC

tyrosine aminotransferase

AT2G20610

Regulation of cell growth by extracellular stimulus, glucosinolate biosynthetic process, adventitious root development, indoleacetic acid biosynthetic process

[6]

NPTII

AAL92039

F: CCGGTATAAAGGGACCACCT

R: ATGTTGCTGTCTCCCAGGTC

neomycin phosphotransferase II

Neomycin phosphotransferase II as selectable markers in transformed plant screening

[81]

Actin

XM_004306544

F: GGCCGTTCTCTCTCTGTATGC

R: TTCTGGGCACCTGAATCTC

Actin

Actin

[29]

FvRD29A

XM_011472069

F: AGAGGCACGCCACGAATA

R: GCCAGTTTGGTCCTTGCT

Cold regulated protein

AT5G52310

Responded to abscisic acid, cold, desiccation, mannitol, osmotic stress, reactive oxygen species, salt stress, water deprivation, and wounding

[34]

FvNCED3

XM_004300619

F: TCACCACAAGAGATTCCTTTCT

R: ATGGCTGTGAGTAGGTTGGA

Encodes 9-cis-epoxycarotenoid dioxygenase

AT3G14440

Regulated in response to drought and salinity

[32]

FvDREB2A

XM_004307642

F: TGCTGCGAGTCTACTACGATGTCT

R: TGATCCATAGGAACCTCGCTTT

Encodes a transcription factor binds to DRE/CRT cis elements

AT5G05410

Regulates expression of many water stress–inducible genes.

[35]

FvABA8

XM_004300635

F: ATGGAACTACTCAGCCTCAACT

R: ATGGCGAGCGGAAATGAG

Encodes a protein with ABA 8′-hydroxylase

AT4G19230

Involved in ABA catabolism.

[46]

FvABI1

XM_011469096

F: GCATGGCTAAAGCTAGTAACAGAC

R: GCTCCTCCAGTGTTATCTTCTTGT

Protein phosphatase 2C family protein

AT4G26080

Involved in abscisic acid (ABA) signal transduction

[33]

FvPYL9

XM_004306638

F: GGGAGTCTTAGGGAAGTGAATG

R: GGATGGACGGTGATAATAGAAGAG

Encodes regulatory components of ABA receptor

AT1G01360

Interacts with and regulates the type 2C protein phosphatases (PP2Cs).

[78]

FvPP2C

XM_004291463

F: ACGAAATATGCCGAGAAGCGAAAG

R: TTGGCGGAAATCGTTAGGCTCAT

Encodes a member of the group A protein phosphatase 2C

At1g07430

Negative regulation of abscisic acid-activated signaling pathway.

[36]

FvAAO1

XM_004296223

F: TCAACCGCTGCTCCATCACG

R: GTCGGTCAGTGGTGTTTTGGGC

Encodes aldehyde oxidase 1

AT5G20960

Involved abscisic acid biosynthetic process, auxin biosynthetic process.

[76]

FvPIN1

XM_004303589

F: CAAAGCCGCAAAGATAGAGCC

R: TGGGATGCCCTGACCTGAT

Encodes an auxin efflux carrier involved in shoot and root development

AT1G73590

Involved in the maintenance of embryonic auxin gradients

[82]

FvARF7

XM_004287612

F:ACAATCACTGGCATTAGCGAGC

R: GAGGTGGGCAGATGTAGAAAGG

Encodes an auxin-regulated transcriptional activator

AT5G20730

Involved in auxin-activated signaling pathway, lateral root development, lateral root formation.

[83]

FvMAX2

XM_011468656

F:ACTGTGGCGATTTGACGGAT

R: CCACAACAAAGAAGCCAGCGT

F-box leucine-rich repeat family of proteins

AT2G42620

Responses to abiotic stress conditions.

[84]

FvYUC1

JF898837

F: CAAATGGTTGGAAAGGTGAGC

R: CGAGGACATTGAGCGGTGTTGGA

Encodes a member of the YUC family

AT4G32540

Involved in auxin biosynthetic process.

[72]

FvYUC3

JX417080

F: ATTTCCCAACCTACCCAACC

R: AGTCTTGACGAGCCAAAGTC

Encodes a member of the YUC family

AT1G04610

Involved in auxin biosynthetic process, oxidation-reduction process, response to ethylene.

[72]

FvGA3ox

XM_004302902

F: CCTGTAAGAATCTCCGAAGCC

R: CGTGTAGTGAGTTGAAGTCTGC

gibberellin 3-beta-dioxygenase activity,

AT1G15550

Involved in later steps of the gibberellic acid biosynthetic pathway.

[85]

  1. aF forward primer, R reverse primer. Underlined characters indicate the restriction enzyme cutting site