An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar

Table 1 Generation of stably transformed plants from transiently transformed leaves

	Number of explantsa	Explants forming positive calli (%)^b	Positive calli producing transgenic plants (%)^c	Transformation frequency (%)^d	No. of plants
Super:GFP	37	54.1	75.0	40.5	15
35S:GUS	23	95.7	75.0	65.2	15
35S:GUS	30	96.6	66.7	66.6	20
Total	90	–	–	–	50

^a The sum of explants;
^b The percentage of explants that formed positive calli with shoot and leaf primordia;
^c The percentage of positive calli that produced at least one transgenic rooted shoot;
^d The percentage of explants that regenerated at least one transgenic rooted shoot

ISSN: 1471-2229