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Fig. 3 | BMC Plant Biology

Fig. 3

From: An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar

Fig. 3

The ideal P. davidiana ×bolleana plant for agroinfiltration and transient expression of reporter gene. a The ideal plant with an age of PI 12 and its optimum leaves of LPI 4 and LPI 5 used for agroinfiltration. The syringe agroinfiltration was conducted using A. tumefaciens GV3101 suspended in modified infiltration medium [10 mM MgCl2, 5 mM MES-KOH (pH 5.6) and 1.6 mM AS]. b Transient expression of green fluorescent protein (GFP) reporter in epidermal cells and mesophyll cells. The expression of GFP was evaluated at 5 dpi. To observe the fluorescent signals in the mesophyll cells, the lower epidermis was removed with a tweezer. The upper two rows show GFP expression in the epidermal cells with different magnitudes of enlargement. The third row shows GFP expression in the mesophyll cells

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BMC Plant Biology

ISSN: 1471-2229

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