Table 2 A list of primers used to amplify different deletions of the EcGS1b gene promoter
Primer | Sequence(5′-3′) |
|---|---|
GS1bSp1 | GAGAGTGTCCGTGCTTTGCTTCT |
GS1bSp2 | GCTTGAGCCATCATAGTTCCAC-3 |
GS1bSp3 | TCCCCTTGTGGTGTGTAGCAATCGC-3 |
F66 | 5′-CCCAAGCTTCATATCCCACCACCGCAT-3’ |
F340 | 5′-CCCAAGCTTCAGTGTCTGTCAGC-3’ |
F635 | 5′-CCCAAGCTTATTCATACATCTACAATC-3’ |
F838 | 5′-CCCAAGCTTCCATTGGCTGAGAATGG-3’ |
R1257 | 5′-CGGGATCCGGTTGATAAGGTCTGTG-3’ |
qPCR-F | 5′-TTCAGGGTGACTGGAATGG-3’ |
qPCR-R | 5′-TCCAACACGGATTGATGCT-3’ |
Eact-F | 5′-CATTCAATGTGCCTGCCATGT-3’ |
Eact-R | 5′-GGATAGCATGTGGAAGGGCATAG-3’ |