Fig. 4

Effects of overexpression and RNAi interference of MdNAT7 on salt tolerance in apple. a. Western blot assays of MdNAT7 expression in WT, Oe and Ri plants. b. Phenotype of apple plants after salinity stress for 12 days. c. Net photosynthesis rates. d. Total chlorophyll. e. Concentration of MDA. f. Relative electrolyte leakage. g–h. Changes in X and UA concentrations in apple leaves under salinity stress. Three-month-old seedlings of transgenic and WT plants were irrigated with 200 mM NaCl for 12 days, and the leaves were used for physiological analyses. Different letters indicate significant differences between treatments, according to Tukey’s multiple range tests (P<0.05)