Transcriptomic profiling of wheat near-isogenic lines reveals candidate genes on chromosome 3A for pre-harvest sprouting resistance

Table 4 qRT-PCR primers and results

Gene	Direction	Sequence (5′→3′)	NIL	Mean relative expression at 15 DPA	Mean relative expression at 25 DPA	Mean relative expression at 35 DPA
TraesCS3A01G461400	Forward	TTGCCGCTACTCAACAAGCC	R	16.20**	12.99**	11.09**
TraesCS3A01G461400	Reverse	TCTGTTGTCTTCGCCGACT	S	8.47	7.95	5.25
TraesCS3A01G462000	Forward	CATATCATTCGCAAATCCCT	R	8.97*	5.76*	4.23*
TraesCS3A01G462000	Reverse	TCTTCTGGCCTTACCCAA	S	7.09	4.54	3.01
TraesCS3A01G466700	Forward	CCCTGCAAGCCTCGAAAGCAT	R	5.36*	5.07*	4.25*
TraesCS3A01G466700	Reverse	AACCTGCTCGCCATCCGTGA	S	5.98	5.81	5.03
TraesCS3A01G459200	Forward	CTTCCTTGAGCTCTCCTTCAAC	R	6.71*	6.44*	5.83
TraesCS3A01G459200	Reverse	GCAGACTTGCTGAGGAACAT	S	5.58	5.50	5.33
TraesCS3A01G245000	Forward	TGGGACTCTTGTTCTCGCTCT	R	3.42	3.72*	2.98*
TraesCS3A01G245000	Reverse	TCATCGTGCCAGAATGTGACC	S	3.41	4.48	3.58
TraesCS3A01G225100	Forward	ACGTTCAGTTTCCACACGGGTC	R	12.73**	9.26	7.72
TraesCS3A01G225100	Reverse	GCCCAACTCCATACCGATCGAGA	S	9.71	9.47	7.49
Actin	Forward	CTCCCTCACAACAACCGC
Actin	Reverse	TACCAGAACTTCCATACCAAC

*, significant difference (p ≤ 0.05); **, highly significant difference (p ≤ 0.01) between ‘R’ (resistant) and ‘S’ (susceptible) isolines, based on t-test; mean relative expression is the mean of NIL1 and NIL2; expression data are shown in ΔCt values, calculated by subtracting the Ct number of the reference gene (Actin) from that of the target gene

ISSN: 1471-2229