Fig. 2

Comparison of NIL pairs 1 and 2. A. Phenotypic differences between resistant (R) and susceptible (S) isolines in the two NIL pairs. (a) and (b) were the spike sprouting test of NIL pair 1 (left) and NIL pair 2 (right), respectively, at day 7 of the test; (c) and (d) were seed germination test at day 2 of the test. B. Venn diagrams showing the number of differentially expressed genes (DEGs) that were commonly (a) up-regulated and (b) down-regulated in the resistant isolines compared with those in the susceptible isolines. Numerals inside the parentheses indicate the number of genes expressed at each time point. The total number of DEGs is noted at the bottom of each Venn diagram. C. Volcano plot showing DEGs within each NIL pair at different time-points. X axis represents log2 transformed fold change. Y axis represents -log10 transformed p value significance. Blue points represent up-regulated DEGs. Red points represent down-regulated DEGs. Gray points represent non-DEGs. DPA = days post anthesis