Data collection | ZmDRIK1-KD apo | ZmDRIK1-KD + ENMD-2076 |
---|---|---|
X-ray source | APS BEAMLINE 24-ID-E | DIAMOND BEAMLINE I24 |
Wavelength (Ã…) | 0.979180 | 0.976230 |
Space group | P 21 21 21 | P 21 2 21 |
Cell dimensions (Ã…) a, b, c (Ã…) | 61.49 61.5144.76 | 60.51 61.79 65.38 |
Resolution (Å) | 19.82–1.7 | 28.9–2.0 |
No. of unique reflections | 58,167 (2974) | 17,144 (1238) |
Rmerge (%) | 6.4 (203) | 5.5 (87.1) |
Mean I/σI | 16.4 (1.62) | 14.6 (2.0) |
Completeness (%) | 99.9 (99.9) | 99.9 (100) |
Redundancy | 10.9 (10.7) | 6.4 (6.8) |
CC 1/2 | 0.99 (0.519) | 0.99 (0.72) |
Refinement | ||
 Resolution range (Å) | 19.82–1.7 | 28.9–2.0 |
 R/Rfree (%) | 19.9/21.5 | 20.81/25.08 |
 Mean B-factor (Å) | 35.0 | 48.0 |
 r.m.s.d. bond lengths (Å) | 0.0066 | 0.0072 |
 r.m.s.d. bong angles (degrees) | 1.10 | 1.11 |
Ramachandran plot statistics (%) | ||
 Preferred regions | 99.05 | 99.61 |
 Outlier | 0.0 | 0.0 |
PDB ID | 6CYP | 6EAS |
Crystallization conditions | 0.15 M potassium bromide, 30% PEG2000 MME | 1 mM zinc chloride; 22% PEG6000;100 mM MES; pH 6.0 |