Fig. 5
From: Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber
Analysis by qRT-PCR of the effect of BR, SNAP, tungstate and BRz on the expression of NR gene in cucumber explants. Explants were incubated for 24 h in water (control) or in the presence of the different compounds in concentration as indicated on the figure. Relative expression was determined using the ΔΔCt method [37], taking actin gene as a reference. Data were collected from 3 independent biological replicates. Each replicate represents a pool of ten explants. Bars denoted by identical letters did not differ significantly (Duncan’s multiple test; P < 0.05)