Fig. 4

Effects of BR, SNAP, L-NAME, Tungstate and BRz on the content of NO and BR-induced NOS-like and NR activities. The fluorescence localization of NO in hypocotyl of cucumber explants (A) treated with control (distilled water) (a), 1 μM BR(b), 50 μM SNAP (c), BR + 20 μM L-NAME (d) and BR + 100 μM Tungstate (e), 1.5 μM BRz (f) for 24 h. Fluorescence intensity was analyzed using Image J software and expressed as a percentage of the control (B). The quantification of endogenous NO content was done in hypocotyl of cucumber explants (C). NOS-like (D) and NR (E) enzymatic activities were measured from 1 cm-long segment taken from the base of hypocotyl. Mean and SE values were calculated from three independent experiments (n = 15). Bars denoted by the same letter did not differ significantly at P < 0.05 according to Duncan’s multiple test