Fig. 3

Effect of BR on endogenous NO content, NOS-like and NR activities in a time- dependent manner. The primary root system was removed from the hypocotyls of 5-d-old germinated cucumber seedlings. Enzymatic activities were measured from 1 cm-long segment taken from the base of hypocotyl. NO levels (a) were determined by Griess reaction reagent in hypocotyls of explants treated with distilled water (control) or 1 μM BR, 1.5 μM BRz. NOS-like (b) and NR activities (c) of hypocotyls (1 cm) were determined using the NOS-like and NR determination Kit (Jiancheng, Nanjing, China) according to the manufacturer’s instructions in explants treated with distilled water (control) or 1 μM BR,1.5 μM BRz. Values (means ± SE) are the averages of three independent experiments (n = 15 explants from each of three independent experiments). Asterisks indicate that mean values are significantly different between treatments and control (P < 0.05) according to Duncan’s multiple test