Fatty acids | Name of fatty acids | 0 day (Stage I) | 4th day (Stage II) | 8th day (Stage III) | 12th day (Stage IV) | 16th day (Stage IV) | 20th day (Stage V) |
---|---|---|---|---|---|---|---|
Lipid composition | |||||||
 ∑ SFA |  | 49.71 ± 1.46 | 30.12 ± 1.98 | 25.66 ± 0.45 | 28.43 ± 0.65 | 31.14 ± 1.25 | 33.65 ± 2.86 |
 ∑ MUFA |  | 2.31 ± 0.72 | 1.96 ± 0.01 | 1.84 ± 0.01 | 1.73 ± 0.08 | 2.07 ± 0.13 | 3.16 ± 0.93 |
 ∑ PUFA |  | 47.98 ± 0.74 | 67.92 ± 1.99 | 72.51 ± 0.44 | 69.84 ± 0.73 | 66.79 ± 1.38 | 63.18 ± 3.80 |
 ∑ 18 PUFA |  | 47.61 ± 0.75 | 66.78 ± 2.20 | 70.61 ± 0.81 | 68.19 ± 0.85 | 65.4 ± 1.85 | 61.48 ± 4.96 |
 ∑ 20 PUFA |  | 0.37 ± 0.01 | 1.14 ± 0.21 | 1.90 ± 0.37 | 1.64 ± 0.13 | 1.39 ± 0.46 | 1.70 ± 1.17 |
 UI |  | 119.25 ± 0.51 | 174.56 ± 3.91 | 188.25 ± 0.91 | 180.57 ± 0.09 | 171.52 ± 3.75 | 153.78 ± 8.15 |
 DU |  | 98.26 ± 2.21 | 137.8 ± 3.97 | 146.85 ± 0.88 | 141.41 ± 1.38 | 135.64 ± 2.63 | 129.53 ± 6.66 |
 n6/n3 |  | 1.3 ± 0.24 | 1.10 ± 0.05 | 1.01 ± 0.01 | 1.00 ± 0.09 | 1.05 ± 0.06 | 2.10 ± 0.17 |
 n9/n3 |  | 0.11 ± 0.04 | 0.06 ± 0.01 | 0.05 ± 0.01 | 0.04 ± 0.01 | 0.06 ± 0.01 | 0.14 ± 0.06 |
 n9/n6 |  | 0.08 ± 0.02 | 0.06 ± 0.01 | 0.05 ± 0.01 | 0.04 ± 0.01 | 0.05 ± 0.01 | 0.06 ± 0.03 |
 AI |  | 31.29 ± 3.11 | 38.72 ± 0.39 | 39.72 ± 0.79 | 38.14 ± 0.54 | 38.86 ± 0.77 | 48.01 ± 0.93 |
 TI |  | 97.73 ± 4.25 | 120.52 ± 0.48 | 129.41 ± 0.67 | 128.12 ± 3.31 | 122.46 ± 2.25 | 87.19 ± 4.95 |
Fatty acids composition | |||||||
 C12:0 | Lauric acid | n.d | n.d | n.d | n.d | 0.77 ± 0.59 | 0.63 ± 0.21 |
 C14:0 | Myristic acid | 0.27 ± 0.01 | 0.17 ± 0.02 | 0.16 ± 0.02 | 0.24 ± 0.01 | 0.29 ± 0.01 | n.d |
 C14:1 | Myristoleic acid | n.d | n.d | n.d | n.d | n.d | 0.14 ± 0.02 |
 C15:0 | Pentadecanoic acid | 0.20 ± 0.01 | 0.18 ± 0.01 | 0.2 ± 0.02 | 0.23 ± 0.01 | 0.26 ± 0.01 | 0.22 ± 0.02 |
 C16:0 | Palmitic acid | 32.53 ± 0.73 | 22.61 ± 0.39 | 20.23 ± 0.28 | 21.96 ± 0.11 | 23.68 ± 0.97 | 24.53 ± 0.46 |
 C16:1 (n-7) | Palmitoleic acid | n.d | n.d | 0.19 ± 0.01 | 0.18 ± 0.02 | 0.24 ± 0.01 | 0.27 ± 0.02 |
 C17:0 | Heptadecanoic acid | 0.18 ± 0.01 | 0.16 ± 0.01 | 0.18 ± 0.01 | 0.20 ± 0.02 | 0.25 ± 0.01 | 0.23 ± 0.01 |
 C17:1 (n-7) | Cis-10-Heptadecanoic acid | n.d | n.d | n.d | n.d | n.d | 0.06 ± 0.02 |
 C18:0 | Stearic acid | 15.31 ± 0.91 | 5.37 ± 1.62 | 3.41 ± 0.61 | 4.15 ± 0.55 | 4.44 ± 0.60 | 6.55 ± 2.48 |
 C18:1 (n-9) | Oleic acid | 2.14 ± 0.72 | 1.47 ± 0.07 | 1.41 ± 0.05 | 1.34 ± 0.10 | 1.65 ± 0.13 | 2.65 ± 0.97 |
 C18:2 (n-6) | Linoleic acid | 26.88 ± 2.46 | 30.66 ± 2.02 | 30.82 ± 0.44 | 30.38 ± 2.00 | 30.66 ± 0.30 | 38.84 ± 2.31 |
 C18:3 (n-6) | gamma-Linolenic acid | 20.73 ± 1.16 | 4.58 ± 0.19 | 4.72 ± 0.21 | 3.80 ± 0.01 | 3.16 ± 0.31 | 2.16 ± 0.43 |
 C18:3 (n-3) | alpha-Linolenic acid [ALA] | n.d | 31.54 ± 0.48 | 35.07 ± 0.69 | 34.02 ± 1.03 | 31.57 ± 1.97 | 20.49 ± 2.11 |
 C20:0 | Arachidic acid | 0.67 ± 0.04 | 0.76 ± 0.03 | 0.71 ± 0.01 | 0.74 ± 0.06 | 0.70 ± 0.06 | 0.52 ± 0.01 |
 C20:1 (n-9) | Cis-11-Eicosenoic acid | 0.17 ± 0.01 | 0.50 ± 0.06 | 0.23 ± 0.04 | 0.21 ± 0.01 | 0.19 ± 0.01 | 0.04 ± 0.01 |
 C20:2 | Cis-11,14-Eicosadienoic acid | 0.11 ± 0.02 | 0.51 ± 0.03 | 0.28 ± 0.03 | 0.30 ± 0.01 | 0.25 ± 0.04 | 0.10 ± 0.01 |
 C20:3 (n-6) | Cis-8,11,14-Eicosatrienoic acid | n.d | n.d | 0.74 ± 0.42 | 0.47 ± 0.22 | 0.29 ± 0.37 | 1.61 ± 1.01 |
 C20:3 (n-3) | Cis-11,14,17-Eicosadienoic acid | 0.26 ± 0.16 | 0.63 ± 0.06 | 0.87 ± 0.01 | 0.87 ± 0.01 | 0.85 ± 0.12 | n.d |
 C21:0 | Heneicosanoic acid | n.d | n.d | n.d | 0.05 ± 0.01 | 0.04 ± 0.02 | 0.05 ± 0.01 |
 C22:0 | Behenic acid | 0.33 ± 0.01 | 0.52 ± 0.05 | 0.43 ± 0.02 | 0.42 ± 0.05 | 0.38 ± 0.05 | 0.27 ± 0.02 |
 C23:0 | Tricosanoic acid | n.d | n.d | n.d | 0.10 ± 0.01 | n.d | 0.07 ± 0.01 |
 C24:0 | Lignoceric acid | 0.23 ± 0.01 | 0.35 ± 0.06 | 0.32 ± 0.02 | 0.34 ± 0.03 | 0.31 ± 0.05 | 0.18 ± 0.02 |