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PnPht1;1
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F: ATGTCTGGGAATAATCTGCAGG
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to amplify cDNA ORF
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R: CTAAACTTGTGCCTCATTTGCA
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PnPht1;2
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F: ATGGCTCGAGAGCAACTAGAAG
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R: TTATACCGGCACAGTCCTGTTAG
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qPnPht1;1
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F:GAACGGGAATTTTGGTTGCTG
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to amplify segments for qPCR
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R:AAGTCGCCTTGTGGCTGAGTG
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qPnPht1;2
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F:GCGCGTTTATAGCGGCTGTTT
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R:ACTTTTGCCTCTTGGGGGTCA
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26S-2(reference gene)
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F:CAGTATTTAGCCTTGGACGGAATT
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R:CGGGTTGTTTGGGAATGC
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SPnPht1;1(BamH I/Kpn I)
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F: CGCGGATCCATGTCTGGGAATAATCTGCAGG
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to amplify genes for recombinant plasmid construction in S. cerevisiae
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R: CGGGGTACCCTAAACTTGTGCCTCATTTGCA
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SPnPht1;2(Xba I/ Xma I)
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F: TGCTCTAGAATGGCTCGAGAGCAACTAGAAG
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R:TCCCCCCGGGTTATACCGGCACAGTCCTGTTAG
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NbPnPht1;1(BamH I/Sal I) pENTR
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F:GAGAACACGGGGGACTGGTACCCGGGGATCCATGTCTGGGAATAATCTGCAGG
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to amplify genes for recombinant plasmid construction in N. benthamiana
|
|
R:ACAGCTCCTCGCCCTTGCTCACCATGTCGACAACTTGTGCCTCATTTGCATCCT
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NbPnPht1;2(BamH I/Sal I) pENTR
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F:GAGAACACGGGGGACTGGTACCCGGGGATCCATGGCTCGAGAGCAACTA
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R:ACAGCTCCTCGCCCTTGCTCACCATGTCGAC ACGAATGGCCCTTTTATCC
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NbUPF0057(BamH I/Sal I) pENTR
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F:GAGAACACGGGGGACTGGTACCCGGGGATCCATGGTCTCAAGATGTGCA
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R:ACAGCTCCTCGCCCTTGCTCACCATGTCGACAGCAAGAGTGTCATAACG
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