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Table 1 Specific primer pairs used for ORF cloning, qPCR, and recombinant plasmid construction. 26S-2 was used as an internal control genes

From: Phosphate transporters, PnPht1;1 and PnPht1;2 from Panax notoginseng enhance phosphate and arsenate acquisition

GenesPrimers(5′-3′)Usage
PnPht1;1F: ATGTCTGGGAATAATCTGCAGGto amplify cDNA ORF
R: CTAAACTTGTGCCTCATTTGCA
PnPht1;2F: ATGGCTCGAGAGCAACTAGAAG
R: TTATACCGGCACAGTCCTGTTAG
qPnPht1;1F:GAACGGGAATTTTGGTTGCTGto amplify segments for qPCR
R:AAGTCGCCTTGTGGCTGAGTG
qPnPht1;2F:GCGCGTTTATAGCGGCTGTTT
R:ACTTTTGCCTCTTGGGGGTCA
26S-2(reference gene)F:CAGTATTTAGCCTTGGACGGAATT
R:CGGGTTGTTTGGGAATGC
SPnPht1;1(BamH I/Kpn I)F: CGCGGATCCATGTCTGGGAATAATCTGCAGGto amplify genes for recombinant plasmid construction in S. cerevisiae
R: CGGGGTACCCTAAACTTGTGCCTCATTTGCA
SPnPht1;2(Xba I/ Xma I)F: TGCTCTAGAATGGCTCGAGAGCAACTAGAAG
R:TCCCCCCGGGTTATACCGGCACAGTCCTGTTAG
NbPnPht1;1(BamH I/Sal I) pENTRF:GAGAACACGGGGGACTGGTACCCGGGGATCCATGTCTGGGAATAATCTGCAGGto amplify genes for recombinant plasmid construction in N. benthamiana
R:ACAGCTCCTCGCCCTTGCTCACCATGTCGACAACTTGTGCCTCATTTGCATCCT
NbPnPht1;2(BamH I/Sal I) pENTRF:GAGAACACGGGGGACTGGTACCCGGGGATCCATGGCTCGAGAGCAACTA
R:ACAGCTCCTCGCCCTTGCTCACCATGTCGAC ACGAATGGCCCTTTTATCC
NbUPF0057(BamH I/Sal I) pENTRF:GAGAACACGGGGGACTGGTACCCGGGGATCCATGGTCTCAAGATGTGCA
R:ACAGCTCCTCGCCCTTGCTCACCATGTCGACAGCAAGAGTGTCATAACG