Fig. 5
Complementation assays of MB192 cells expressing PnPht1;1 and PnPht1;2. a Growth curves of WT, MB192, MB192-YEplac112, MB192-PnPht1;1, and MB192-PnPht1;2 for culturing 40 h in the presence of a low-Pi concentration (20 μM). b Medium color change with pH caused by acid phosphatase (ACP) activity under different Pi concentrations. c ACP activity of the cells in the presence of low Pi (20 μM) and adjusting the initial pH to 6. Different lowercase letters represent the difference of ACP activity among cells, P ≤ 0.05. d The effect of varying the pH of the medium on the growth of WT, MB192, MB192-YEplac112, MB192-PnPht1;1, and MB192-PnPht1;2 supplement with 100 μM Pi. Different lowercase letters represent the difference of OD600 under the same pH among cells, P ≤ 0.05. e The growth of cells expressing PnPht1;1 or PnPht1;2 was suppressed by protonophores, carbonyl cyanide m-chlorophenylhydrazone (CCCP) and 2,4-dinitrophenol (DNP). ** represents the difference of the same yeast cell between the control and each treatment, P ≤ 0.01. Error bars indicate mean values ± SD, (n = 4)