Fig. 3

Subcellular localization of fusion proteins of PnPht1;1- and PnPht1;2- GFP. GFP-fusion proteins were transiently expressed in Nicotiana benthamiana using agroinfiltration of EHA105 and localized 4–6 days after infiltration. The N. tabacum UPF0057 membrane protein (XM_016579128.1) acted as a membrane marker. Merge, subcellular localization pictures of fusion protein through merging membrane marker and GFP. Yellow parts in the merge indicated that fusion proteins localized in the plasma membrane. The scale bar in each picture is 32 μm