Reducing the size of an alien segment carrying leaf rust and stripe rust resistance in wheat

BMC Plant Biology

Table 4 Segregation of 6B-chromotypes in BC₃ and BC₄ populations

Line	BC₃F₂ population					BC₄F₂ population
Line	Total No. of plants	No. of non- recombinants	No. of heterozygotes	No. of recombinant homozygotes^a	Χ² *	Total No. of plants	No. of non- recombinants	No. of heterozygotes	No. of recombinant homozygotes^a	Χ² *
R-1	48	15	25	8 (12)	2.13	39	5	28	6 (9.75)	7.46*
R-2	33	11	18	4 (8.25)	3.24	23	8	13	2 (5.75)	3.52
R-3	33	9	20	4 (8.25)	3.00	26	7	17	2 (6.5)	4.38
R-4	33	16	16	1 (8.25)	13.67*	25	8	13	4 (6.25)	1.32
R-16	33	11	22	0 (8.25)	11.00*	19	5	14	0 (4.75)	6.89*
R-7	50	10	28	12 (12.5)	0.88	26	4	16	6 (6.5)	1.69
R-18	34	7	16	11 (8.5)	1.06	25	10	15	0 (6.25)	9.00*
R-19	33	14	16	3 (8.25)	7.36*	32	7	17	8 (8)	0.19
R-10	33	12	9	12 (8.25)	6.82*	25	7	7	11(6.25)	6.12*
R-20	33	10	17	6 (8.25)	1.00	25	9	14	2 (6.25)	4.28
P-37	31	8	19	4 (7.75)	2.61	25	6	13	6 (6.25)	0.04

PCR marker Zyg_1Sh_1 was used to determine presence of Ae. sharonensis resistance segment, markers Zyg_1Sh_1 and Zyg_2G_2 determined zygosity of the plants. BC₄ was produced by pollinating heterozygous BC₃ by cv. Galil
*Significant (p > 0.05) deviation from the expected distribution
^aValues in brackets denote the expected number of recombinant homozygotes

ISSN: 1471-2229