Fig. 6From: Identification of tissue-specific and cold-responsive lncRNAs in Medicago truncatula by high-throughput RNA sequencingResponses of lncRNA MtCIR1 and their potential target MtCBF genes to cold treatment. a: Transcriptional responses of MtCIR1 in leaves of M. truncatula seedlings subjected to cold treatment. b: Transcriptional responses of MtCBF genes in leaves of M. truncatula seedlings subjected to cold treatment. Leaf RNA was extracted from 3-week-old seedlings cold treated at 4 °C for varying time periods and then the relative expression was detected using real-time quantitative RT-PCR. Values are means ± SE (n = 3) and the asterisks indicate significant differences at p < 0.05 according to t-test relative to room temperature control (0 h)Back to article page