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Fig. 5 | BMC Plant Biology

Fig. 5

From: Identification of tissue-specific and cold-responsive lncRNAs in Medicago truncatula by high-throughput RNA sequencing

Fig. 5

Relationship between lncRNA MtCIR1 and their potential target MtCBF genes in M. truncatula plants. Transcriptional responses of MtCIR1 (a) and MtCBF genes (b) in M. truncatula plants to cold treatment at 4 °C for 5 h analyzed by RNA-seq (TPM). Transcription responses of MtCIR1 (c) and MtCBF genes (d) in M. truncatula plants to cold treatment at 4 °C for 5 h verified by real-time quantitative Q-PCR. NT: non-cold treated; CT: cold treated; L: leaves; R: roots. Values are means ± SE (n = 3) and the asterisks indicate significant differences at p < 0.05 between cold-treated sample (CT) and non-cold-treated control (NT) according to t-test. e: Genome map of the MtCBF gene clusters and MtCIR1. MtCIR1 and MtCBF gene clusters are located in a 145.3 kb on chromosome 6 of M. truncatula Jemalong A17. Red rectangle indicates exon. Red arrow indicates exon and direction of gene from 5′ to 3′. Green line indicates intron. Blue rectangle indicates lncRNA. Black line indicates intergenic region. Black numbers indicate length (bp) of intergenic region

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