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Fig. 7 | BMC Plant Biology

Fig. 7

From: Barley ROP-Interactive Partner-a organizes into RAC1- and MICROTUBULE-ASSOCIATED ROP-GTPASE ACTIVATING PROTEIN 1-dependent membrane domains

Fig. 7

YFP-RIPa localization at sites of fungal attack by Bgh. a Whole cell Z-stack images were taken 28 h after biolistic transformation of barley epidermal cells and 23 h after inoculation. The MT-marker RFP-MAGAP1-Cterm was co-expressed to visualize MTs. Bars represent 20 μm. Additionally, untagged RAC1 and untagged MAGAP1 are co-expressed. b Same cell as in A imaged at a higher zoom factor. Brightness was enhanced by 40% after imaging. Please note the fungal attack from an appressorium (app). YFP-RIPa is visible in membrane patches and around the site of attack (arrow). n, plant nucleus. Bars represent 8 μm

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