Fig. 6

Functional domains of MAGAP1 determine the formation of YFP-RIPa membrane domains. a Domain architecture of MAGAP1 and of truncated versions of MAGAP1, which were expressed as RFP fusion proteins. b Co-expression of fluorescent YFP-RIPa (shown in green) with untagged RAC1 (or without RAC1; outer right column) with three versions of RFP-MAGAP1 (shown in magenta) with or without ROP-binding and MT-association domains as depicted in a. Whole cell Z-stack images were taken 24 h after biolistic transformation of barley epidermal cells. Bars represent 20 μm