Fig. 5

Disturbance of YFP-RIPa localization to MT-restricted domains of the cell periphery/plasma membrane. YFP-RIPa (shown in green) when co-expressed with wild type RAC1 and MAGAP1 can be found in MT-restricted domains of the cell periphery/plasma membrane (see left panel for DMSO solvent control). This localization is dissolved when MTs are destroyed by either 10 or 30 μM oryzalin treatment (solved in 0.25% [v/v] DMSO, treated for 3.2 h before imaging). Whole cell Z-stack images were taken 24 h after biolistic transformation of barley epidermal cells. The MT-marker RFP-MAGAP1-Cterm (shown in magenta) was co-expressed to visualize MTs. Bars represent 30 μm