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Fig. 3 | BMC Plant Biology

Fig. 3

From: Barley ROP-Interactive Partner-a organizes into RAC1- and MICROTUBULE-ASSOCIATED ROP-GTPASE ACTIVATING PROTEIN 1-dependent membrane domains

Fig. 3

Single cell expressed barley YFP-RIPa changes subcellular localization upon co-expression of untagged RAC1. YFP-RIPa alone (Ø) localizes to immobile speckles and the cytoplasm. Co-expression of untagged RAC1 (WT RAC1) leads to plasma membrane and MT association of YFP-RIPs, co-expression of CA RAC1 leads to plasma membrane localization of YFP-RIPa and DN RAC1 leads to speckle-association of YFP-RIPa. The lower panels show digital magnifications of the YFP-RIPa signals with 40% enhanced brightness. Whole cell Z-stack images were taken 24 h after biolistic transformation of barley epidermal cells. The MT-marker RFP-MAGAP1-Cterm was co-expressed to visualize MTs. Bars represent 20 μm in the upper row and 5 μm in the detail magnifications

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BMC Plant Biology

ISSN: 1471-2229

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