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Fig. 7 | BMC Plant Biology

Fig. 7

From: Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)

Fig. 7

Identification of RopGEF mutants using T-DNA insertion and CRISPR-Cas9 system. a Scheme of the DNA structure and mutant regions. In the gene structure figures, the black boxes are exon region, black lines are intron region, and the arrow indicates the position of mutation occurs. Red letters indicate the target site of sgRNA, and black box indicate the PAM site in front of the sgRNA target sites. Ins; insertion, del; deletion. b Fertility ratio comparison between wild-type and ropgef2/8 mutant plants. Error bars are standard deviation of more than three panicles in the plants. Phenotype of plant growth (c) Bar = 10 cm, and (d) flower structures, Bar = 2 mm, (e) reproductive organs formation, Bar = 2 mm, (f) pollen starch accumulation stained with KI, Bar = 20 μm, (g) pollen wall exine staining using auramin O, (h) pollen wall intine staining using calcoflour white, Bar = 20 μm, (i) In-vitro pollen germination test in solid pollen media, Bar = 250 μm, (j) enlarged pollen pictures, Bar = 10 μm, (k) Pollen germination ratio in vitro environment. Black bar indicates wild-type pollen; grey bar indicates RopGEF2/8 mutant pollen. Error bars are standard deviation of three technical repeats

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