Table 6 Differentially expressed miRNAs between HN and LN treatments and their predicted target gene functions

Known

bdi-miR156b-5p

19

81

4.27E-02

–

–

–

–

–

tae-miR159a-p5

18

38

1.75E-02

–

–

–

–

–

osa-miR159a.1_L + 1

–

–

–

–

–

41

85

1.39E-02

MYB; WD40 repeat-like protein

ata-miR164a-5p

–

–

–

–

–

19

54

4.90E-02

glutathione S-transferase; MAP kinase;

ata-miR169d-5p

–

–

–

–

–

7

23

2.13E-02

protein serine/threonine kinase; ABC transporter C family member 13

ata-miR169d-3p_L-2R + 2

–

–

–

–

–

27

92

8.22E-05

galactinol--sucrose galactosyltransferase 2; E3 ubiquitin-protein ligase

ata-miR172b-5p

–

–

–

–

–

43

2

1.38E-03

–

sbi-miR399a

27

113

5.40E-03

9

109

1.05E-03

–

–

Oxidoreductase; transcription factor

hvu-miR399

–

–

–

24

54

4.73E-03

–

–

UDP-glucosyltransferase; transcription factor

ata-miR528-5p

–

3

23

2.20E-02

lectin-rich repeat receptor kinase

osa-miR530-5p_R + 1_1ss20AG

5

31

3.22E-02

0

11

2.57E-03

–

–

–

osa-miR530-5p_L + 1

–

–

–

–

–

0

30

4.62E-03

fructose-bisphosphate aldolase; SCF ubiquitin ligase complex

osa-miR827

–

–

–

174

566

2.36E-05

–

–

spx-domain-contain; Phenylalanyl-tRNA synthetase

tae-miR9653a-3p

–

–

–

–

–

181

0

9.46E-04

–

tae-miR9659-3p

55

0

6.03E-03

_

_

_

_

–

tae-miR9661-5p_1ss14GA

–

–

–

_

_

22

0

5.49E-03

–

tae-miR9664–3p_L-1

15

0

2.00E-02

29

62

4.34E-03

55

151

2.29E-03

RPM1; Gibberellin receptor; peptidyl-prolyl cis-trans isomerase;

tae-miR9666a-3p

–

–

–

–

–

4812

911

1.38E-07

Alternative splicing regulator; regulatory protein NPR5-like

tae-miR9666b-3p

–

–

–

–

–

674

318

2.57E-04

stress response

tae-miR9672a-3p_L + 2R-2

–

–

–

15

41

4.01E-02

19

5

5.50E-02

replication factor C subunit 1

tae-miR9774_L + 2

–

–

–

–

–

107

42

1.12E-03

–

tae-miR9777_L + 2

–

–

–

–

–

14

46

2.94E-02

geraniol 8-hydroxylase-like; SCF ubiquitin ligase

Novel

PC-3p-40666_99

32

0

1.06E-02

–

–

–

–

transcription factor activity

PC-5p-11080_759

72

21

1.73E-02

–

–

0

30

3.60E-03

–

PC-5p-26413_215

23

4

3.03E-02

23

9

4.43E-02

11

0

5.99E-03

BRI 1-associated receptor kinase 1-like; LRR receptor-like serine/threonine-protein kinase

PC-3p-43479_87

24

5

4.85E-02

–

–

–

–

–

tae-miR9655-p5

2936

668

2.87E-04

–

–

–

–

oxidation-reduction process; transcription factor

PC-5p-16963_436

–

32

91

8.60E-04

0

74

1.78E-02

–

tae-miR1137a-p3_1ss9CT

–

9

25

1.92E-02

–

–

–

PC-3p-15988_474

–

–

–

206

51

9.29E-04

peroxidase activity

PC-5p-6533_1330

–

–

–

269

47

3.16E-03

regulation of transcription

PC-3p-33976_137

–

–

–

25

0

3.29E-03

RPM1

PC-5p-21478_303

–

–

–

77

21

2.41E-02

lectin S-receptor-like kinase

PC-5p-36040_123

–

–

–

24

9

3.25E-02

protein disulfide isomerase; endopeptidase

tae-miR9666a-p5

–

–

–

101

30

3.22E-05

–

tae-miR2916-p3_2ss2TC17CA

–

–

–

27,736

7049

1.51E-03

fructose-bisphosphate aldolase; PRM1; peroxidase

tae-miR2916-p5_2ss2AG17TG

–

–

–

9557

2868

5.04E-03

glutathione S-transferase; DELLAL

tae-miR2592bj-p3_2ss12TC19AT

–

–

–

380

135

1.72E-02

protein serine/threonine kinase activity; RPM1;

PC-3p-21085_312

–

–

–

3

44

1.36E-03

–

PC-3p-21502_303

–

–

–

0

25

1.97E-03

metalloendopeptidase activity; MYB

PC-5p-9458_913

–

–

–

0

133

3.25E-03

–

PC-5p-4529_1828

–

–

–

62

144

1.15E-02

–

PC-3p-11233_746

–

–

–

9

33

2.87E-02

metalloendopeptidase activitys

tae-miR9783-p3_2ss5AG18AC

–

–

–

4

59

4.41E-04

protein RPM1-like

tae-miR9657c-p5_1ss20TC

–

–

–

17

114

5.39E-04

Leucine-rich receptor-like protein kinase;

tae-miR5384-p5

–

–

–

132

489

5.66E-04

UDP-glycosyltransferase; transcription factor

tae-miR9779-p3

–

–

–

11

93

2.83E-03

hexose transmembrane transport

tae-miR815c-p5_2ss6CT21CT

–

–

–

21

51

4.56E-03

UDP-arabinose 4-epimerase; TGA2-like; Dnaj

tae-miR1127b-p5_1ss3TC

–

–

–

0

20

6.05E-03

–

tae-miR1122c-p3

–

–

–

17

46

4.34E-02

phosphatase HAL2; protein kinase