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Table 5 Confirmation of the expression profiles of selected genes by qRT-PCR

From: Transcriptome profiling of differentially expressed genes in cytoplasmic male-sterile line and its fertility restorer line in pigeon pea (Cajanus cajan L.)

Gene Id

Description

Fold change

  

RNA-Seq

qRT-PCR

Ccajan_44775_c0_g1_i1

callose synthase 7-like

−5.4

−4.4

Ccajan_34307_c0_g1_i1

phosphoenolpyruvate carboxykinase

−2.2

−1.7

Ccajan_83966_c0_g1_i1

Polygalacturonase (PG)

−8.9

−6.6

Ccajan_24040_c0_g3_i1

aspartic protease

10.4

8.2

Ccajan_52697_c0_g1_i1

Late embryogenesis abundant protein 6 (LEA6)

−4.5

−6.3

Ccajan_12415_c0_g2_i1

uncharacterized mitochondrial protein

−12.3

−8.1

Ccajan_36196_c0_g2_i3

glutathione s-transferase F10

2.41

2.0

Ccajan_47280_c0_g1_i1

calmodulin binding receptor-like protein

−7.5

−5.8

Ccajan_51888_c0_g1_i1

mitotic spindle assembly checkpoint protein (MAD1)

−4.6

−3.9

Ccajan_47116_c0_g1_i1

pentatricopeptide repeat-containing protein

−2.5

−1.2

  1. Fold change refers to expression in sterile AKCMS11 in comparison to restorer AKPR303; Negative value refers down-regulated expression in sterile AKCMS11