Table 2 Summary of Illumina sequencing and de novo transcriptome
Read processing | |||
|---|---|---|---|
| Â | AKCMS11 (two replicates) | AKPR303 (two replicates) | Total |
Raw reads | 109,600,365 | 72,575,988 | 182,176,353 |
Clean reads (after trimming) | 101,676,579 | 69,481,226 | 171,157,805 |
Average read length (bp) | 100 | 100 | Ë— |
Trinity based de novo assembly | |||
No. of transcripts (n) | 1,98,587 | ||
 Percentage GC | 41.14 | ||
 Contig N10 (bp) | 3148 | ||
 Contig N20 (bp) | 2300 | ||
 Contig N30 (bp) | 1788 | ||
 Contig N40 (bp) | 1375 | ||
 Contig N50 (bp) | 1009 | ||
 L50 value | 6 | ||
 Median contig length | 357 | ||
 Average length | 641.34 | ||
 Total assembled bases | 127,362,415 | ||
Unigenes | |||
 Total number of unigenes (n) | 1,71,095 | ||
 Average length (bp) | 561.66 | ||
 N50 length (bp) | 757 | ||
 Total assembled bases | 97,193,132 | ||