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Fig. 7 | BMC Plant Biology

Fig. 7

From: Higher growth of the apple (Malus × domestica Borkh.) fruit cortex is supported by resource intensive metabolism during early development

Fig. 7

Spatiotemporal patterns of the major storage organic acids, malate (a) and quinate (b), and transcript abundance of malate metabolism-related genes (c-f) in apple fruit in response to fruit load reduction. Metabolite concentration was determined using gas chromatography and transcript abundance was measured using quantitative RT-PCR. CC: Control fruit load-Cortex; CP: Control fruit load-Pith; RC: Reduced fruit load-Cortex; RP: Reduced fruit load-Pith. Fruit load reduction was performed at 11 d after full bloom. Mean and S.D. (n = 4) are presented for metabolite data. The mean and S.E. of the mean (n = 4) are displayed for transcript abundance. Asterisk and dagger symbols indicate significant difference between control and reduced fruit load treatments in the cortex and pith, respectively (α = 0.05). PEPC: PHOSPHOENOLPYRUVATE CARBOXYLASE; MDH: MALATE DEHYDROGENASE. All expression data are presented in reference to mean expression at 0 d after treatment in RC. Transcript abundance of a target gene was normalized to that of apple ACTIN and GAPDH (GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE) genes. Shaded regions in the background indicate early (dark grey), mid (light grey), and late fruit development (white) periods

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