Fig. 7From: Higher growth of the apple (Malus × domestica Borkh.) fruit cortex is supported by resource intensive metabolism during early developmentSpatiotemporal patterns of the major storage organic acids, malate (a) and quinate (b), and transcript abundance of malate metabolism-related genes (c-f) in apple fruit in response to fruit load reduction. Metabolite concentration was determined using gas chromatography and transcript abundance was measured using quantitative RT-PCR. CC: Control fruit load-Cortex; CP: Control fruit load-Pith; RC: Reduced fruit load-Cortex; RP: Reduced fruit load-Pith. Fruit load reduction was performed at 11 d after full bloom. Mean and S.D. (n = 4) are presented for metabolite data. The mean and S.E. of the mean (n = 4) are displayed for transcript abundance. Asterisk and dagger symbols indicate significant difference between control and reduced fruit load treatments in the cortex and pith, respectively (α = 0.05). PEPC: PHOSPHOENOLPYRUVATE CARBOXYLASE; MDH: MALATE DEHYDROGENASE. All expression data are presented in reference to mean expression at 0 d after treatment in RC. Transcript abundance of a target gene was normalized to that of apple ACTIN and GAPDH (GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE) genes. Shaded regions in the background indicate early (dark grey), mid (light grey), and late fruit development (white) periodsBack to article page