Table 1 Throughput and quality of strand-specific RNA-seq of F. tataricum libraries
From: Transcriptome profiling of Fagopyrum tataricum leaves in response to lead stress
| Â | CK | Pb1 | Pb2 |
|---|---|---|---|
HiSeq Statistics | |||
 Raw reads | 48,264,504 | 51,792,400 | 47,292,888 |
 Raw base (bp) | 7,287,940,104 | 7,820,652,400 | 7,141,226,088 |
  > Q20 of raw data (%) | 97.07 | 97.29 | 96.84 |
 Clean reads | 47,737,814 | 51,208,144 | 46,731,827 |
 Clean base (bp) | 7,126,704,866 | 7,613,422,269 | 6,959,839,129 |
  > Q20 clean reads (%) | 97.68 | 97.91 | 97.50 |
 GC percentage (%) | 50.48 | 53.15 | 49.01 |
Assembly data (all the clean reads of the 6 libraries were assembled together) | |||
 Total unigene number | 88,977 | ||
 Total unigene base | 125,203,555 | ||
 Percent GC | 41.27 | ||
 Length of the largest unigene (bp) | 19,818 | ||
 Length of the smallest unigene (bp) | 201 | ||
 Average length | 982.34 | ||
 N50 | 1730 | ||
 E90N50 | 1942 | ||