Fig. 1

Schematic illustration of target design and the binary vector. a Schematic map of the target sites within VvCCD7 and VvCCD8 genes. The sequences of sgRNAs are indicated in red. CCD7-F/R and CCD8-F/R are primers used for PCR amplification. b Schematic diagram of the revised pCACRISPR/Cas9 vector. The EGFP reporter gene was used for rapid selection of transformed cells after transformation. 35S, CaMV35S promoter; AtU6, Arabidopsis small RNA U6 promoter; T_NOS, nopaline synthase terminator; RB, right border; LB, left border