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Table 1 Treatment temperatures of leaves for different measurements. Freezing temperatures were chosen according to previously reported LT50 values of − 5.34 °C NA and − 9.68 °C ACC [28]

From: Repair of sub-lethal freezing damage in leaves of Arabidopsis thaliana

MethodNon-acclimatedCold acclimated
Samples frozen toSamples frozen to
non-lethal temperature (°C)lethal temperature (°C)non-lethal temperature (°C)lethal temperature (°C)
Electrolyte leakage− 3.0, − 3.5, − 4.5, − 5− 7− 8, − 8.5− 11
Chlorophyll fluorescence imaging− 5, − 6, − 7− 8− 9, − 10, − 11− 12, − 13
Respiration− 6, − 7− 8− 8, − 9− 12
Sugar content− 4.5, − 5, − 6, − 7− 8− 8, − 8.5, − 9, − 10, − 11− 12, − 13
Proline content− 4.5, − 5, − 6, − 7− 8− 8, − 8.5, − 9, − 10, − 11− 12, − 13
Gene expression analysis (qRT-PCR)− 5, − 6, − 7− 8− 9, − 10, − 11,− 12, − 13