Fig. 3

Respiration rates measured as oxygen consumption in unfrozen Arabidopsis leaves (UFC, 1D-UFC, 3D-UFC, 6D-UFC) and in leaves frozen to different temperatures right after thawing (RAT) or after recovery for 1 day (1D-REC), 3 days (3D-REC) or 6 days (6D-REC) at 4 °C for a, b non-acclimated plants and c, d cold acclimated plants. a, c Respiration was measured at the recovery temperature (4 °C); b, d respiration was measured at room temperature (21 °C). Data are the means of five replicates with two leaf discs each with standard errors. Significance levels between values measured at UFC/RAT and at the subsequent time points determined by Student’s t-test are indicated as: ***, p < 0.001, **, p < 0.01, *, p < 0.05