Lipases of germinating jojoba seeds efficiently hydrolyze triacylglycerols and wax esters and display wax ester-synthesizing activity

Table 1 Wax ester synthesis by the microsomal fractions of germinated jojoba seeds from different substrates

	Substrates added (1 nmol of each one/assay)
	[¹⁴C]18:1-OH			Tri-[¹⁴C]18:1-TAG			18:1-OH
	–	+ 18:1-DAG	+ 18:1-TAG	–	+ 18:1-OH	+ cholesterol	+ [¹⁴C]18:1-FA	+ [¹⁴C]18:1-CoA	+ [¹⁴C]PC	+ [¹⁴C]PE
[¹⁴C]WE formed [pmol]	167 ± 19	284* ± 27	248* ± 22	76 ± 13	163* ± 7	61 ± 6	65 ± 9	0.0	0.0	0.0
[¹⁴C]WE formed [% of control]	100	159	139	100	214	80	-	-	-	-

^* – significant difference between control assays with only [¹⁴C] substrate added and assays with two substrates (t test, p < 0.01, n = 4)
Data represent the mean of four biological replicates ± standard deviation. Assay conditions: aliquots (2.5 nmol of endogenous PC) of microsomal fractions of germinated jojoba seed (35 dpg); substrates added to freeze dried microsomes in 19 μl benzene; benzene evaporation and addition of 100 μl 0.1 M phosphate buffer (pH 7.2); 15 min incubation at 35 °C

ISSN: 1471-2229