Fig. 5

Clustal Omega alignment of Exon 8 (highlighted in grey) and upstream intronic region of the A, B and D homoeoalleles of SSIIa gene in the truncation mutants. The A and D genome common G > A mutation, and the B genome C > T mutation leading to predicted truncation in the three homoeoalleles have been highlighted in red. The position of genome-specific forward primers is depicted in bold and underlined (A genome: green, B genome: blue, D genome: brown), and common reverse primer is bold and underlined in black