Fig. 1

Molecular analysis of transgenic chickpea lines a: PCR analyses of four transgenic chickpea events (T₀); b: PCR analyses of transgenic chickpea progenies (T₁) derived from E₅; c: PCR analyses of transgenic chickpea progenies (T₁) derived from E₁₇; d: PCR analyses of transgenic chickpea progenies (T₁) derived from E₁₉; e: PCR analyses of transgenic chickpea progenies (T₁) derived from E_22; [L1–100 bp DNA ladder and L2–1 kb DNA ladder]; f: Southern blot analysis (L: DIG-labelled DNA ladder; I-IV: Four independent transgenic chickpea lines E₅, E₁₇, E₁₉ and E₂₂ (T1 stage); N: Non-transformed chickpea (DCP 92–3); P: Positive control (Binary plasmid). g: RT-PCR analysis (L1: 1Kb plus DNA ladder; P: Positive control; N: Negative control; I-IV: Transgenic chickpea lines (T₁ stage); V–X: Transgenic chickpea lines (T₂ stage); NTC: No Template Control; C: RNA as Template; L2: 100 bp DNA ladder) [Mean SM 11.8% and mean LWP −0.82 MPa]