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Table 2 List of oligonucleotides for Yeast One-Hybrid (Y1H) assay and electrophoretic mobility shift assay (EMSA)

From: EgJUB1 and EgERF113 transcription factors as potential master regulators of defense response in Elaeis guineensis against the hemibiotrophic Ganoderma boninense

Target Motifs

DNA element sequences (5′ - 3′)

NACBS

GATGCCGTGATGCCGTGATGCCGTGATGCCGT

mNACBS

GATGACGTGATGACGTGATGACGTGATGACGT

SNBE1

TTGTGTGCTGTGGAAGTTTTTGTGTGCTGTGGAAGTTT

mSNBE1

TTGTGGGCTGTGGAAGTTTTTGTGGGCTGTGGAAGTTT

SNBE2

TGGCTTGTGCAAAAAGTAATGGCTTGTGCAAAAAGTAA

mSNBE2

TGGCTCGTGCAAAAAGTAATGGCTCGTGCAAAAAGTAA

GCC-box

TAAGAGCCGCCTAAGAGCCGCCTAAGAGCCGCCTAAGAGCCGCC

mGCC-box

TAAGATCCTCCTAAGATCCTCCTAAGATCCTCCTAAGATCCTCC

DRE/CRT

TGCCGACATTGCCGACATTGCCGACATTGCCGACAT

mDRE/CRT

TATTTACATTATTTACATTATTTACATTATTTACAT

  1. The tandem repeats of DNA elements of each target are bold and underlined. Point mutations are bold and underlined with red
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BMC Plant Biology

ISSN: 1471-2229

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