Fig. 3

EgJUB1 interacts with secondary wall NAC binding element (SNBE) during defense response against biotrophic infection. a Yeast One-Hybrid analysis reveals interaction of EgJUB1 with tandem repeats of SNBE1 motif. Negative interaction was observed with both NAC binding site (NACBS) and SNBE2 motifs. Transformed yeast cells were cultured on SD/−Leu/AbA^†, wherein ^† denotes optimized concentration of antibiotic Aureobasidin A (AbA) for each motif. b EMSA shows direct binding of EgJUB1 to SNBE1 motif. c Prediction of binding motifs for EgJUB1 derived from Plant Transcription Factor Database (PlantTFDB) version 5.0. http://planttfdb.cbi.pku.edu.cn/. d Putative SNBE sequences identified from 1.5-kb promoters of EgJUB1 direct targets. The number shown on the left of each sequence is the position of the first nucleotide relative to the start codon. The plus or minus symbol on the right of each sequence indicates first nucleotide of SNBE sequences from the forward or reverse strand of the target’s DNA. ‡ indicates SNBE1 core motif and § indicates SNBE2 core motif tested in the study. ¶ represents SNBE1 core motif used in the present study, with single nucleotide change. X is defined as an isoform