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Fig. 11 | BMC Plant Biology

Fig. 11

From: Genome-wide identification and analysis of DNA methyltransferase and demethylase gene families in Dendrobium officinale reveal their potential functions in polysaccharide accumulation

Fig. 11

Transcript levels of DoC5-MTase and DodMTase genes, as well as the genes involved in the biosynthesis of WSPs, at different developmental stages. a The expression patterns of DoC5-MTase and DodMTase genes during different developmental stages. b Proposed biosynthetic pathway of WSPs in D. officinale. c The expression patterns of genes involved in the biosynthesis of WSPs during different developmental stages. The three developmental stages are protocorm-like bodies (S1), shoots (S2) and plantlets (derived from protocorm-like bodies, S3), which were collected at 2, 4 and 10 months after germination, respectively. The heat map was generated by log2 transformation of the average of the expression values of D. officinale DoC5-MTase and DodMTase genes during different developmental stages. Blue and red in the color scale indicate low and high transcript expression, respectively. In C, error bars indicate the mean ± SD (standard deviation) of three individual experiments, and were quantified with three independent replicates. Different lowercase letters above bars indicate a significant difference among different stages at p < 0.05 based on Duncan’s multiple range test. CSLA, cellulose synthase-like A; Fru, fructose; Gal, galactose; Glc, glucose; GMP, GDP-mannose pyrophosphorylase; Man, mannose; PMM, phosphomannomutase; UGE, UDP-glucose epimerase; UGP, UDP-glucose pyrophosphorylase; WSP, water-soluble polysaccharide

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