Fig. 6
From: GmbZIP1 negatively regulates ABA-induced inhibition of nodulation by targeting GmENOD40–1 in soybean
GmbZIP1 overexpression inhibits soybean nodulation. a qRT-PCR analysis of GmbZIP1 expression in transgenic empty or UBQ::GmbZIP1 roots at 7 DAI. Values are mean ± SD, p< 0.001. b Infection foci of individual hairy roots expressing the empty vector and UBQ::GmbZIP1. At 7 DAI, 2-cm root segments of hairy roots overexpressing GmbZIP1 below the root-hypocotyl junction were cut and stained with 1% (w/v) methylene blue. Considerably deformed root hairs were counted. The asterisk marks the curled root hair; c Quantitative analysis of the infection foci number per hairy root expressing empty vector or UBQ::GmbZIP1 at 7 DAI. Values are mean ± SD, p< 0.001. d qRT-PCR analysis of GmbZIP1 expression in transgenic empty vector or UBQ::GmbZIP1 roots at 14 DAI. Values are mean ± SD, p< 0.001. e, f Overexpression of GmbZIP1 decreased the number of nodulation. The nodule number was counted at 14 DAI of empty vector and UBQ::GmbZIP1