Fig. 5
From: GmbZIP1 negatively regulates ABA-induced inhibition of nodulation by targeting GmENOD40–1 in soybean
GmbZIP1 is responsive to ABA. a qRT-PCR analysis of expression of GmbZIP1 in the response to ABA. Five-day-old seedlings of soybean were treated with 20 μM ABA for three hours. GmELF1b was used as an endogenous control for gene expression. The expression levels are shown as the means ± SDs from three replicates. b The subcellular localization analysis of GmbZIP1. GmbZIP1 was fused to YFP and expressed in Arabidopsis. c Germination rates of wild type and GmbZIP1 overexpression line on MS. d Germination rates of wild type and GmbZIP1 overexpression line on MS supplemented with 0.5 μM ABA. * P< 0.05, *** P< 0.001. e Greening rates of wild type and GmbZIP1 overexpression line on MS. f Greening rates of wild type and GmbZIP1 overexpression line on MS supplemented with 0.5 μM ABA. * P< 0.05