Fig. 2

Network analysis of S6PP tandem duplication genomic region. The Network was built using NETWORK 5 software by Median-joining method, from the alignment of a region of approximately 1539 bp of various domesticated varieties of sugarcane, the species used as parental in the crosses (S. spontaneum and S.officinarum) and sequenced BACs of R570 and SP80–3280. The largest node was zoomed for a better visualization and is shown inside the square. a The largest node is formed by R570 BACs predicted to transcribe a single transcript containing two domains of S6PP, SP80–3280 BACs and 36 sequences from POJ-2878, NCo-310, RB-72454, NA56–79, RB-867515, SP70–1143, Miscanthus, Co-290 and RB-835486 domesticated varieties; b node formed by six sequences; c node formed by nine sequences; d node formed by eight sequences. The smaller nodes are formed by only one sequence. The size of the node (circle) is relative to the number of sequences in that haplotype. The distance between the nodes is proportional to the number of substitutions. (*) Refers to the domesticated varieties used in the study: Mandalay, SP70–1143, Miscanthus, Co-290, RB-835486, POJ-2878, Nco-310, RB-72454, NA 56–79, RB 867515, SP81–3250, R570, SP80–3280