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Fig. 4 | BMC Plant Biology

Fig. 4

From: Characterization of type-2 diacylglycerol acyltransferases in Haematococcus lacustris reveals their functions and engineering potential in triacylglycerol biosynthesis

Fig. 4

Heterologous expression of HpDGAT2D in Chlamydomonas reinhardtii cells. a Construct of the expression vector. PsaD-P, promoter of PsaD gene; His tag, 6-His encoding gene; PsaD-T, terminator of PsaD gene; RBCS2-P, promoter of RBCS2 gene; Ble, the bleomycin-resistant gene; RBCS-T, terminator of RBCS gene. b Genomic level detection of HpDGAT2D in C. reinhardtii cells. c Time course of biomass content under control and 1/4‚ÄČN conditions. d The transcriptional expression levels of HpDGAT2D in C. reinhardtii cells. The gene expression levels were normalized to the endogenous actin gene. e Western blotting of HpDGAT2D-His tag fusion protein with His-tagged antibodies. Soluble and membrane proteins were separated and used for blotting. Actin which was known soluble protein was used as controls. f TAG contents and relative abundance of fatty acid in C. reinhardtii cells transformed with HpDGAT2D

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