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Table 3 Segregation of genotypes at DNA markers for QTLs and associated rate of preharvest sprouting

From: Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

Marker name plant no. plant number of each genotype Ratio of pre-harvest sprouting (%) One-way ANOVA
Population AA AB BB AA AB BB F-value P
qPHS1_KY29_PHS_17515
 Cross A_1 130 29 69 32 23.3 ± 23.3 10.9 ± 18.1 4.5 ± 7.1 9.14 0.0020 *
 Cross A_2 106 21 62 23 11.2 ± 16.4 9.1 ± 12.3 6.2 ± 6.5 0.93 0.4137
qPHS6_KY29_WG_26859
 Cross A_1 130 35 59 36 25.5 ± 26.8 8.4 ± 13.4 8.7 ± 12.6 7.13 0.0057 *
 Cross A_2 106 20 61 25 9.5 ± 9.6 10.1 ± 13.9 5.4 ± 9.3 1.35 0.2865
qPHS1_KY28_Sh_3180
 Cross B_1 100 40 42 18 59.4 ± 30.9 69.0 ± 24.5 69.4 ± 25.5 1.51 0.2483
 Cross B_2 106 34 49 23 60.6 ± 28.5 58.8 ± 25.4 55.0 ± 23.2 0.32 0.7309
qPHS3_KY28_WG_1572
 Cross B_1 100 27 49 24 77.7 ± 24.7 67.0 ± 25.8 47.7 ± 25.8 9.03 0.0021 *
 Cross B_2 106 34 52 20 70.1 ± 21.3 57.5 ± 25.2 41.8 ± 23.0 8.75 0.0024 *
qPHS4_KY28_PHS_921
 Cross B_1 100 26 50 24 78.4 ± 21.6 67.4 ± 27.1 46.7 ± 25.0 10.21 0.0012 *
 Cross B_2 106 22 59 25 57.0 ± 26.3 61.5 ± 25.6 53.0 ± 26.1 0.99 0.3906
qPHS8_KY28_WG_21775
 Cross B_1 100 18 49 33 50.5 ± 30.7 60.7 ± 27.6 80.1 ± 17.8 9.48 0.0017 *
 Cross B_2 106 23 57 26 43.5 ± 19.2 57.2 ± 26.8 75.0 ± 19.5 11.01 0.0009 **
  1. A, KSC7 allele; B, KY29 or KY28 allele
  2. *P < 0.05; **P < 0.001