Targeted amplicon sequencing + next-generation sequencing–based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)

Table 2 QTLs for preharvest sprouting

Populations	QTLs	LG	Closest marker	peak position (cM)	LOD	Additive effect^b	Dominant effect	R2 (%)^c
Populations	QTLs	LG	(position (cM))	peak position (cM)	LOD	Additive effect^b	Dominant effect	R2 (%)^c
Cross A_1	qPHS1_KY29	1	Sh_11581 –PHS_17515	71.2	4.08	10.29	−0.48	11.7
	qPHS1_KY29	1	(69.0)–(71.2)	71.2	4.08	10.29	−0.48	11.7
	qPHS6_KY29	6	WG_1684 – WG_26859	59.5	4.54	12.21	−7.44	15.6
	qPHS6_KY29	6	(36.5)–(59.5)	59.5	4.54	12.21	−7.44	15.6
	qPHS4_KY29^a	4	WG_657 – PHS_9932	57.8	2.44	8.26	−3.5	7.4
	qPHS4_KY29^a	4	(52.8)–(58.8)	57.8	2.44	8.26	−3.5	7.4
	qPHS7_KY29^a	7	WG_982 – WG_7916	49.6	2.35	−7.05	−3.3	7.5
	qPHS7_KY29^a	7	(46.6)–(57.7)	49.6	2.35	−7.05	−3.3	7.5
Cross B_1	qPHS1_KY28	1	Sh_11090 – Sh_3180	45	3.54	−9.18	6.45	9
	qPHS1_KY28	1	(43.2)–(45.0)	45	3.54	−9.18	6.45	9
	qPHS3_KY28	3	PHS_31886 – WG_1572	3.5	4.09	12.78	5.61	10.6
	qPHS3_KY28	3	(2.9)–(3.5)	3.5	4.09	12.78	5.61	10.6
	qPHS4_KY28	4	PHS_921 – PHS_10421	5.2	7.48	17.28	−0.36	22
	qPHS4_KY28	4	(5.2)–(7.6)	5.2	7.48	17.28	−0.36	22
	qPHS8_KY28	8	WG_21775 – WG_1135	7	5.58	−14.83	−1.84	15.7
	qPHS8_KY28	8	(6.0)–(8.3)	7	5.58	−14.83	−1.84	15.7
	qPHS5_KY28^a	5	WG_119 – WG_16811	47.6	2.82	−5.91	11.62	7.1
	qPHS5_KY28^a	5	(44.6)–(52.4)	47.6	2.82	−5.91	11.62	7.1
Cross C	qPHS3_NF1	3	WG_1572 – PHS_12618	53.2	3.47	4.44	4.26	12
	qPHS3_NF1	3	(51.9)–(53.2)	53.2	3.47	4.44	4.26	12
	qPHS8_NF1	8	WG_6029 –WG_1135	12.1	4.49	−4.16	4.64	16
	qPHS8_NF1	8	(8.1)–(15.5)	12.1	4.49	−4.16	4.64	16
	qPHS2_NF1^a	2	WG_74 – WG_1243	24.1	3.06	3.58	4.35	10
	qPHS2_NF1^a	2	(13.1)–(24.1)	24.1	3.06	3.58	4.35	10

^aMinor QTLs
^bEffect contributed by KSC7 alleles
^cPercentage of total variation in marker association for each trait across population explained by QTL

ISSN: 1471-2229