Fig. 5

Overexpression of VvNPF6.5 enhances nitrate accumulation and NUE in Arabidopsis. a and b, Plants were grown hydroponically for 24 days and treated with 2.25 mM K¹⁵NO₃ for 30 min. ¹⁵N contents in shoots (a) and roots (b) were analyzed. c, Plants were grown hydroponically for 24 days and shoots and roots were harvested to analyze the dry biomass and total N. Values are means ± SD, n = 5. Asterisks indicate difference between wild type and overexpression lines at P < 0.05 (*) and P < 0.01 (**) by Student’s t-test